Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Browse for different fluorescent readers below and compare across different instrument suppliers. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. Plus 384. Part Number. Place the microplate in the drawer. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. When a. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data. Selects either 96-well or 384-well plate for a stand-alone read. 2 OD at 200 nm; this decreased to 0. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. 2. 00. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). (Molecular Devices cat. Product Compare 0. Plus 384. Site. Click on the crosshair and move it so that it is centered over the well image. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. On the day of the assay, the plate was equilibrated. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. . B. Figure 1-2 Match chamfered corners of slide and adapter 2. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Temperature control: 4°C above ambient to 45°C. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 0200-6262. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. x. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Page 1 of 8 . The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. 003 fmol 0. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. A = 2 - log 10 (%T). Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Order Now. 000 to 2. 2. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Microplate readers, SpectraMax®, with SpectraDrop. Published Dec 16,. VMax . Conventional technique: subjective, time-consuming. Important things to consider when purchasing a multimode microplate reader. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Absorbance cuvette port. 0200-6263. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. 1% in white 96- and < 0. 2. 5 OD at 492 nm Computer interface USB 2. 40266. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. The color touch screen provides a visual user interface, making programming fast and intuitive. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Cross-talk <0. ~ Bio-Tek ELx808 Microplate Reader $2,795. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Measurements for every well are automatically tracked over time. Service parts for these models are not being produced and are no longer in stock. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The device was able to achieve 3σ limits of detection of ca. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. U-bottom: 30-40 µl. Bay Gray powder coated metal back plate, column, and base. Published Dec 16, 2020 | Updated Dec 16, 2020. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 2. Compare. The soil was amended with either 1. These models can no longer be repaired. What are the dimensions of the plate? Code. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Includes PC and software. 5 fg per well to 0. adjust the reader. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. * Note: Programming the washer with a final aspirate height of 4. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Z. Fluorescence Microplate Reader Comparison Chart. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. 057, depending on lot number. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. 96-well) while others have flexibility to. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. Please refer to the Obsolete Microplate Reader Models for more information. This reader also makes use of a unique temperature-independent methodology for path length correction. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Figure 2-23: Bottle holder To install the bottle holder: 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. #ABS PLUS) Methods. Loading Buffer was also added to a set of cell-free wells for background controls. 20 attomol ATP per well. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. , Corning, NY) or Greiner. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. FlexIII. UV Plate 96 Well, Flat Bottom, Cs 50. Quantitative, whole well cell confluence for every well. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Microplates, cuvettes (via adapter) Detectors. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Published Dec 16, 2020 | Updated Dec 06, 2021. Page 1 of 8 . and glow luminescence modes in 96- to 384-well plates. 96- or 384-well microplate compatibility. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. Absorbance Linearity: 2. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. 384-WELL SBS STERILE MICROPLATE. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. ©2022 Molecular Devices - All rights. Sort By: Show:. Published Dec 16, 2020 | Updated Dec 16,. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. QC tools and IQOQ services. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Part of a plate can be selected so that only a selected area of the plate. Figure 3. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 4 decades . 1. Luminescence applications and assays. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 300 µL of Detergent Compatible Bradford reagent was added to each well. x. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 00 USD. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Range of 96, 384-well SBS microplates . SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. VIS absorbance detection. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. SoftMax Pro GxP Software version 5. Controlled by the computer. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. 1. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. SpectraMax 250 User. Or scan up to 384 wells at any time to determine the best assay conditions. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Selects either 96-well or 384-well plate for a stand-alone read. The ELx808 TM Reader seamlessly interfaces with Lonza's WinKQCL TM Endotoxin Detection and Analysis Software. Optional 12-way manifold) 5032336. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. 96-well plates - Filter plates with 0. May require LS-50B…. 1 fmol luciferase per well. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. • 0. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Uncontrolled Copy for Training and Reference Purposes Only . UV Plate 96 Well, Flat Bottom, Cs 50. Reads various kinds of 96-wells plates, and is equipped with shaking function. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. The 6-well plates were incubated at 37ºC for 9 days before picking. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. 00. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. 001 ABS (displayed), 0. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. as well as all other Molecular Devices' microplate readers. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. 313 reference gain check fail. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Anticancer drug assay was also conducted using conventional 96-well plates as controls. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. x. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 7°C and the bottom to 36. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. When a. Including injection time, it takes approximately 68 secs. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 314 low lamp level warning. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. These plates are compatible with standard fluorescence microplate readers and are offered in a. 5% at 1. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. 4 (Molecular Devices cat. 2% in. An industry-renowned instrument for monitoring GPCRs and ion channels. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . This page contains the. to each well. Products : General, FilterMax F5 reader. High tolerance for contaminants. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. *For Corning UV plates, mean values at 260 nm ranged from 0. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. 1. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. Cayman Chemical. Automated live cell imaging – cell counting and confluence. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. x. Visit the supplier page or submit a quote to learn more. x. Contents of wells were pipetted up and down several times to mix. 2b,c. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Plate Shaking. 1. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. Versa Max. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. This can increase throughput and efficiency, thus saving time and space in the lab. Our microplate. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 000 OD ± 1. We would like to show you a description here but the site won’t allow us. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. 25% and 0. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. . In addition, the iD5 reader measures TRF and FP and. Endpoint, kinetic, spectrum. #ABS Plus) Cell plating and treatment prior to assay. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. 340 PC384. Microplates, cuvettes (via adapter) Detectors. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. This reader's dual monochromators for. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 020 fmol 0. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 0. com. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. These robust readers have been placed in labs from. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. Figure 2. PURPOSE. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 50/plate. 00 USD. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. 9. Our. tools to expand the boundaries of their research. An industry-renowned instrument for monitoring GPCRs and ion channels. Dimensions: 18. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Next-generation luminescence plate reader. Source plate capacity; 1 x plates. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 1 OD at 230 nm. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Optional 16-way manifold. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. Published Dec 16, 2020 | Updated Dec 16, 2020. 9. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. A. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). 96/384-well plate formats. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. perfect for kinetic readings involving growth curves at 5 seconds per plate. Values are averages from 5 clean plates, each filled with 200 µL water/well. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Create custom fluorescence assays without having to purchase expensive filter sets. Microplate optimization and read height optimization were performed for best results. Plate chamber design of the SpectraMax category 1 microplate readers. This reader can also be used as a standard. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. 304 signal level saturation. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 5032338. Multimodal plate reader: Yes: 25 mm: Yes—Max. Instrument settings can. style. Time-resolved phosphorescence. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Molecular Devices' microplate readers. UV absorbance detection. 96 well plate injection time with SpectraMax® L plate reader. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. robotics-automation-compatible. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Microplate Capacity. Two high-energy xenon flash lamps: Reading capabilities. Component A (2 vials*)Figure 1. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. (96- and 384-well with pipetting) 6-384 (96- and 384-well). Refurbished. Selects either 96-well or 384-well plate for a stand-alone read. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 1 – 2. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. The equation that allows one to calculate absorbance from % transmittance is. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Microplate readers read biological, chemical or physical events that occur on a microtiter plate. minutes and begin reading microplates with seven mouse clicks. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The CellTiter-Glo assay was used to measure ATP. External Dimensions without lid (mm). M2. Access this by going to. #E010) Pyroplate 96-well microplate (ACC cat. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Turn the instrument around so that the back of the instrument is. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. p-Nitroaniline - Catalog No. Wavelength(s):. Contents of wells were pipetted up and down several times to mix. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. Linear, Orbital Slow, and Orbital Fast. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Microplate Readers. 0% ± 0. The top- and bottom-reading Gemini EM Microplate. Molecular Devices SpectraMax Plus 384 Absorbance.